Confocal Calculator
Excitation Wavelength (nm)
405, DAPI, Hoescht
458, CFP
488, GFP, FITC, Cy2
514, YFP
561, 546, TRITC, DiI, Cy3
594, mCherry, Cy3.5
633, 647, Cy5
Emission Wavelength (nm)
Objective Numerical Aperture
Immersion Refractive Index
Air
Water
Glycerol
Oil
Calculate
Resolution
Lateral :
Axial :
Nyquist Sampling
Lateral :
Axial :
Reasonable Sampling
Lateral :
Axial :